Gene profiling and characterization of arginine kinase-1 (MrAK-1) from freshwater giant prawn (Macrobrachium rosenbergii)

Arockiaraj, J.; Vanaraja, P.; Easwvaran, S.; Singh, A.; Alinejaid, T.; Othman, R.Y.; Bhassu, S. (2011) Gene profiling and characterization of arginine kinase-1 (MrAK-1) from freshwater giant prawn (Macrobrachium rosenbergii). Fish & Shellfish Immunology, 31 (1). ISSN 0504648

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Abstract

Arginine kinase-1 (MrAK-1) was sequenced from the freshwater prawn Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique. MrAK-1 consisted of 1068 bp nucleotide encoded 355 polypeptide with an estimated molecular mass of 40 kDa. MrAK-1 sequence contains a potential ATP:guanido phosphotransferases active domain site. The deduced amino acid sequence of MrAK-1 was compared with other 7 homologous arginine kinase (AK) and showed the highest identity (96%) with AK-1 from cherry shrimp Neocaridina denticulate. The qRT-PCR analysis revealed a broad expression of MrAK-1 with the highest expression in the muscle and the lowest in the eyestalk. The expression of MrAK-1 after challenge with the infectious hypodermal and hematopoietic necrosis virus (IHHNV) was tested in muscle. In addition, MrAK-1 was expressed in Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The optimum temperature (30 °C) and pH (8.5) was determined for the enzyme activity assay. MrAK-1 showed significant (P < 0.05) activity towards 10-50 mM ATP concentration. The enzyme activity was inhibited by α-ketoglutarate, glucose and ATP at the concentration of 10, 50 and 100 mM respectively. Conclusively, the findings of this study indicated that MrAK-1 might play an important role in the coupling of energy production and utilization and the immune response in shrimps.

Item Type: Article
Creators:
  1. Arockiaraj, J.
  2. Vanaraja, P.
  3. Easwvaran, S.
  4. Singh, A.
  5. Alinejaid, T.
  6. Othman, R.Y.
  7. Bhassu, S.
Journal or Publication Title: Fish & Shellfish Immunology
Additional Information: Centre for Biotechnology in Agriculture Research, Division of Genetics & Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Arginine kinase-1
Subjects: R Medicine
Divisions: Faculty of Science
Depositing User: Mr. Faizal Hamzah
Date Deposited: 04 Aug 2011 12:19
Last Modified: 11 Dec 2013 10:44
URI: http://eprints.um.edu.my/id/eprint/1932

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