First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties

Ayob, F.W. and Simarani, K. and Abidin, N.Z. and Mohamad, J. (2017) First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties. Microbial Biotechnology, 10 (4). pp. 926-932. ISSN 1751-7915

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Official URL: http://dx.doi.org/10.1111/1751-7915.12603

Abstract

This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography–mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA-MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration (IC50) of > 32 μg ml−1 was observed compared with standard (IC50) of 350 μg ml−1 only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml−1.

Item Type: Article
Uncontrolled Keywords: Antineoplastic Agents; Ascomycota; Catharanthus; Cell Line, Tumor; Cell Survival; Chromatography, Liquid; Formazans; Humans; Inhibitory Concentration 50; Mass Spectrometry; Staining and Labeling; Tetrazolium Salts; Vinblastine
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 18 Sep 2018 01:48
Last Modified: 18 Sep 2018 01:48
URI: http://eprints.um.edu.my/id/eprint/19234

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