Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase

Halim, A.A.A. and Zaroog, M.S. and Kadir, H.A. and Tayyab, S. (2017) Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase. Journal of Saudi Chemical Society, 21 (Spp.1). S349-S358. ISSN 1319-6103

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Official URL: http://dx.doi.org/10.1016/j.jscs.2014.04.002

Abstract

Alcohol-induced structural changes in the acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 were studied by far-ultra violet circular dichroism, intrinsic, three-dimensional and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence, acrylamide quenching and thermal denaturation. All the alcohols used in this study produced partial refolding in the acid-denatured BLA as evident from the increased mean residue ellipticity at 222 nm, increased intrinsic fluorescence and decreased ANS fluorescence. The order of effectiveness of these alcohols to induce a partially folded state of BLA was found to be: 2,2,2-trifluoroethanol/tert-butanol > 1-propanol/2-propanol > 2-chloroethanol > ethanol > methanol. Three-dimensional fluorescence and acrylamide quenching results obtained in the presence of 5.5 M tert-butanol also suggested formation of a partially folded state in the acid-denatured BLA. However, 5.5 M tert-butanol-induced state of BLA showed a non-cooperative thermal transition. All these results suggested formation of a partially folded state of the acid-denatured BLA in the presence of these alcohols. Furthermore, their effectiveness was found to be guided by their chain length, position of methyl groups and presence of the substituents.

Item Type: Article
Uncontrolled Keywords: Acid-denatured state; Alcohol-induced states; Bacillus licheniformis α-amylase; Structural transition; Thermal denaturation
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 07 Sep 2018 02:18
Last Modified: 07 Sep 2018 02:18
URI: http://eprints.um.edu.my/id/eprint/19178

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