Halim, A.A.A. and Zaroog, M.S. and Kadir, H.A. and Tayyab, S. (2017) Alcohol-induced structural transitions in the acid-denatured Bacillus licheniformis α-amylase. Journal of Saudi Chemical Society, 21 (Spp.1). S349-S358. ISSN 1319-6103, DOI https://doi.org/10.1016/j.jscs.2014.04.002.
Full text not available from this repository.Abstract
Alcohol-induced structural changes in the acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 were studied by far-ultra violet circular dichroism, intrinsic, three-dimensional and 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence, acrylamide quenching and thermal denaturation. All the alcohols used in this study produced partial refolding in the acid-denatured BLA as evident from the increased mean residue ellipticity at 222 nm, increased intrinsic fluorescence and decreased ANS fluorescence. The order of effectiveness of these alcohols to induce a partially folded state of BLA was found to be: 2,2,2-trifluoroethanol/tert-butanol > 1-propanol/2-propanol > 2-chloroethanol > ethanol > methanol. Three-dimensional fluorescence and acrylamide quenching results obtained in the presence of 5.5 M tert-butanol also suggested formation of a partially folded state in the acid-denatured BLA. However, 5.5 M tert-butanol-induced state of BLA showed a non-cooperative thermal transition. All these results suggested formation of a partially folded state of the acid-denatured BLA in the presence of these alcohols. Furthermore, their effectiveness was found to be guided by their chain length, position of methyl groups and presence of the substituents.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant (RG160-11AFR), Postgraduate Research Fund (PV018/2011B) |
| Uncontrolled Keywords: | Acid-denatured state; Alcohol-induced states; Bacillus licheniformis α-amylase; Structural transition; Thermal denaturation |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 07 Sep 2018 02:18 |
| Last Modified: | 07 Sep 2018 02:18 |
| URI: | http://eprints.um.edu.my/id/eprint/19178 |
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