CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma

Hoe, S.L.L. and Tan, L.P. and Abdul Aziz, N. and Liew, K. and Teow, S.Y. and Abdul Razak, F.R. and Chin, Y.M. and Mohamed Shahrehan, N.A. and Chu, T.L. and Mohd Kornain, N.K. and Peh, S.C. and Koay, C.E. and Lo, K.W. and Ahmad, M. and Ng, C.C. and Khoo, A.S.B. (2017) CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma. Scientific Reports, 7 (1). p. 12372. ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/s41598-017-12045-8

Abstract

Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, faster-growing tumourigenic cells leading to tumours with larger volume and higher mitotic figures. Although CD44br and EpCAMbr cells significantly enriched for tumour-initiating cells (TICs), all cells could retain self-renewal property for at least four generations. Compared to CD44 marker alone, EpCAM/CD44dbr marker did not enhance for cells with faster-growing ability or higher TIC frequency. Cells expressing high CD44 or EpCAM had lower KLF4 and p21 in NPC subpopulations. KLF4-overexpressed EpCAMbr cells had slower growth while Kenpaullone inhibition of KLF4 transcription increased in vitro cell proliferation. Compared to non-NPC, NPC specimens had increased expression of EPCAM, of which tumours from advanced stage of NPC had higher expression. Together, our study provides evidence that EpCAM is a potentially important marker in NPC.

Item Type: Article
Uncontrolled Keywords: CD24; CD44; EpCAM; Tumour-initiating cells; Patient-derived xenograft; Nasopharyngeal carcinoma
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 06 Sep 2018 06:24
Last Modified: 06 Sep 2018 06:24
URI: http://eprints.um.edu.my/id/eprint/19169

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