Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates

Khatun, M. and Alam, S.M.S. and Khan, A.H. and Hossain, M.A. and Haq, J.A. and Alam Jilani, M.S. and Rahman, M.T. and Karim, M.M. (2017) Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates. Asian Pacific Journal of Tropical Medicine, 10 (8). pp. 753-759. ISSN 1995-7645

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Official URL: http://dx.doi.org/10.1016/j.apjtm.2017.08.002

Abstract

Objective To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions. Test was validated by comparing with several other diagnostic methods. Results The designed primer set showed 100% specificity and 98% sensitivity in detecting VL using blood samples, when compared with more invasive samples: bone marrow or spleen aspirates. Conclusions The newly designed primer MK1F/R could be a better alternative for PCR based diagnosis of VL using less invasive sample, peripheral blood instead of bone marrow or spleen aspirates.

Item Type: Article
Uncontrolled Keywords: Visceral leishmaniasis; Kala-azar; Leishmania species; PCR detection; New primer
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 06 Sep 2018 01:45
Last Modified: 06 Sep 2018 01:45
URI: http://eprints.um.edu.my/id/eprint/19136

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