The uS8, uS4, eS31, and uL14 Ribosomal Protein Genes Are Dysregulated in Nasopharyngeal Carcinoma Cell Lines

Sim, E.U.H. and Ng, K.L. and Lee, C.W. and Narayanan, K. (2017) The uS8, uS4, eS31, and uL14 Ribosomal Protein Genes Are Dysregulated in Nasopharyngeal Carcinoma Cell Lines. BioMed Research International, 2017. pp. 1-8. ISSN 2314-6133, DOI https://doi.org/10.1155/2017/4876954.

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Abstract

The association of ribosomal proteins with carcinogenesis of nasopharyngeal carcinoma (NPC) has been established in a limited subset of ribosomal protein genes. To date, three ribosomal protein genes, eL27 (L27), eL41 (L41), and eL43 (L37a), have been found to be differentially expressed in cell lines derived from NPC tumors. This raises the possibility of more ribosomal protein genes that could be associated with NPC. In this study, we investigated the expression profiles of eight ribosomal protein genes, uS8 (S8), uS4 (S9), eS31 (S27a), eL6 (L6), eL18 (L18), uL14 (L23), eL24 (L24), and eL30 (L30), in six NPC-derived cell lines (HONE-1, SUNE1, HK1, TW01, TW04, and C666-1). Their expression levels were compared with that of a nonmalignant nasopharyngeal epithelial cell line (NP69) using quantitative real-time PCR (RT-qPCR) assay. Of the eight genes studied, the expressions of four ribosomal protein genes uS8 (S8), uS4 (S9), eS31 (S27a), and uL14 (L23) were found to be significantly downregulated in NPC cell lines relative to NP69. Our findings provide novel empirical evidence of these four ribosomal protein genes as NPC-associated genetic factors and reinforce the relevance of ribosomal proteins in the carcinogenesis of nasopharyngeal cancer.

Item Type:	Article
Funders:	Ministry of Higher Education, Malaysia, via the Fundamental Research Grant Scheme [FRGS/ST03(02)/1299/2015(16) and FRGS/ST03(01)/962/2013(03)], Universiti Malaysia Sarawak via the Ph.D. Student Fund [F07(DPP08)/1160/2014(08)]
Uncontrolled Keywords:	Carcinoma; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Nasopharyngeal Neoplasms; Ribosomal Proteins
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Ms. Juhaida Abd Rahim
Date Deposited:	17 Aug 2018 08:00
Last Modified:	17 Aug 2018 08:00
URI:	http://eprints.um.edu.my/id/eprint/19023

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