Cross-species analysis of apical asparagine-rich protein of Plasmodium vivax and Plasmodium knowlesi

Muh, F. and Ahmed, M.A. and Han, J.H. and Nyunt, M.H. and Lee, S.K. and Lau, Y.L. and Kaneko, O. and Han, E.T. (2018) Cross-species analysis of apical asparagine-rich protein of Plasmodium vivax and Plasmodium knowlesi. Scientific Reports, 8 (1). pp. 1-11. ISSN 2045-2322

[img]
Preview
PDF (Open Access)
Cross-species_analysis_of_apical_asparagine-rich_protein_of_Plasmodium_vivax_and_Plasmodium_knowlesi.pdf

Download (1MB)
Official URL: http://dx.doi.org/10.1038/s41598-018-23728-1

Abstract

The Plasmodium falciparum apical asparagine (Asn)-rich protein (AARP) is one of malarial proteins, and it has been studied as a candidate of malaria subunit vaccine. Basic characterization of PvAARP has been performed with a focus on its immunogenicity and localization. In this study, we further analyzed the immunogenicity of PvAARP, focusing on the longevity of the antibody response, cross-species immunity and invasion inhibitory activity by using the primate malaria parasite Plasmodium knowlesi. We found that vivax malaria patient sera retained anti-PvAARP antibodies for at least one year without re-infection. Recombinant PvAARP protein was strongly recognized by knowlesi malaria patients. Antibody raised against the P. vivax and P. knowlesi AARP N-termini reacted with the apical side of the P. knowlesi merozoites and inhibited erythrocyte invasion by P. knowlesi in a concentration-dependent manner, thereby suggesting a cross-species nature of anti-PvAARP antibody against PkAARP. These results can be explained by B cell epitopes predicted in conserved surface-exposed regions of the AARP N-terminus in both species. The long-lived anti-PvAARP antibody response, cross-reactivity, and invasion inhibitory activity of anti-PvAARP support a critical role of AARP during the erythrocyte invasion and suggest that PvAARP induces long-lived cross-species protective immunity against P. vivax and P. knowlesi.

Item Type: Article
Uncontrolled Keywords: Cross-species analysis; Apical asparagine-rich protein; Plasmodium vivax; Plasmodium knowlesi
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 07 Aug 2018 08:10
Last Modified: 07 Aug 2018 08:10
URI: http://eprints.um.edu.my/id/eprint/18979

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year