Fiber density of collagen grafts impacts rabbit urethral regeneration

Larsson, H.M. and Vythilingam, G. and Pinnagoda, K. and Vardar, E. and Engelhardt, E.M. and Sothilingam, S. and Thambidorai, R.C. and Kamarul, Tunku and Hubbell, J.A. and Frey, P. (2018) Fiber density of collagen grafts impacts rabbit urethral regeneration. Scientific Reports, 8 (1). pp. 1-8. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-018-27621-9.

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Abstract

There is a need for efficient and "off-The-shelf" grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts.

Item Type:	Article
Funders:	University Malaya: (UMRG) (RP005G-13HTM and RU028-2015 UM.0000216/HRU.OP), International short-term research fellowship (SNSF: IZK0Z3-171101), Commission of Technology and Innovation of the Swiss Government (CTI project no-16627.1 PFLS-LS)
Uncontrolled Keywords:	Fiber density; Collagen; Grafts; Rabbit urethral regeneration
Subjects:	R Medicine T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Medicine
Depositing User:	Ms. Juhaida Abd Rahim
Date Deposited:	07 Aug 2018 07:57
Last Modified:	10 Oct 2018 09:10
URI:	http://eprints.um.edu.my/id/eprint/18977

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