Yong, Hoi Sen and Song, Sze Looi and Chua, Kah Ooi and Lim, Phaik Eem (2017) Predominance of Wolbachia endosymbiont in the microbiota across life stages of Bactrocera latifrons (Insecta: Tephritidae). Meta Gene, 14. pp. 6-11. ISSN 2214-5400, DOI https://doi.org/10.1016/j.mgene.2017.07.007.
Full text not available from this repository.Abstract
The Solanum fruit fly Bactrocera latifrons is an important agricultural pest. There is no report on the microbiota associated with this fruit fly. This study reports the bacterial communities associated with the life stages of this fruit fly from Malaysia. Illumina MiSeq next-generation sequencing of PCR-generated amplicons of 16S rRNA gene revealed the presence of different number of bacterial OTUs in the life stages studied. The number of bacterial OTUs at different taxonomic levels in the larva sample was much higher than in the adult male and adult female samples. The class Alphaproteobacteria of phylum Proteobacteria was predominant across the life stages studied (98.64% in the larva sample, 98.53% in pupa, 97.66% in adult male, and 99.89% in adult female). At the specific level, Wolbachia endosymbiont of Culex quinquefasciatus Pel was detected in all the life stages studied – 98.61% in the larva sample, 98.18% in pupa, 97.59% in adult male, and 99.88% in adult female. The ubiquitous presence of Wolbachia infections across life stages provides evidence for transmission from the larva stage to emerging adults. The role(s) of Wolbachia in B. latifrons remain to be determined.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya (H-5620009) |
| Uncontrolled Keywords: | Bactrocera latifrons; Wolbachia endosymbiont; Microbiota; Next-generation sequencing |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 29 Jun 2018 08:29 |
| Last Modified: | 04 Dec 2019 08:46 |
| URI: | http://eprints.um.edu.my/id/eprint/18904 |
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