Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Yong, K.W. and Wan Safwani, W.K.Z. and Xu, F. and Zhang, X. and Choi, J.R. and Wan Abas, W.A.B. and Omar, S.Z. and Noor Azmi, M.A. and Chua, K.H. and Pingguan-Murphy, B. (2016) Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells. Journal of Tissue Engineering and Regenerative Medicine, 11 (8). pp. 2217-2226. ISSN 1932-6254

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Official URL: http://dx.doi.org/10.1002/term.2120

Abstract

Cryopreservation represents an efficient way to preserve human mesenchymal stem cells (hMSCs) at early culture/passage, and allows pooling of cells to achieve sufficient cells required for off-the-shelf use in clinical applications, e.g. cell-based therapies and regenerative medicine. To fully apply cryopreserved hMSCs in a clinical setting, it is necessary to evaluate their biosafety, e.g. chromosomal abnormality and tumourigenic potential. To date, many studies have demonstrated that cryopreserved hMSCs display no chromosomal abnormalities. However, the tumourigenic potential of cryopreserved hMSCs has not yet been evaluated. In the present study, we cryopreserved human adipose-derived mesenchymal stem cells (hASCs) for 3 months, using a slow freezing method with various cryoprotective agents (CPAs), followed by assessment of the tumourigenic potential of the cryopreserved hASCs after thawing and subculture. We found that long-term cryopreserved hASCs maintained normal levels of the tumour suppressor markers p53, p21, p16 and pRb, hTERT, telomerase activity and telomere length. Further, we did not observe significant DNA damage or signs of p53 mutation in cryopreserved hASCs. Our findings suggest that long-term cryopreserved hASCs are at low risk of tumourigenesis. These findings aid in establishing the biosafety profile of cryopreserved hASCs, and thus establishing low hazardous risk perception with the use of long-term cryopreserved hASCs for future clinical applications.

Item Type: Article
Uncontrolled Keywords: Biosafety; Clinical applications; Human adipose-derived stem cells; Long-term cryopreservation; Low risk; Tumourigenic potential assessment
Subjects: R Medicine
Divisions: Faculty of Engineering
Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 29 Jun 2018 08:24
Last Modified: 09 Aug 2018 09:14
URI: http://eprints.um.edu.my/id/eprint/18903

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