Yew, S.M. and Chan, C.L. and Ngeow, Y.F. and Toh, Y.F. and Na, S.L. and Lee, K.W. and Hoh, C.C. and Yee, W.Y. and Ng, K.P. and Kuan, C.S. (2016) Insight into different environmental niches adaptation and allergenicity from the Cladosporium sphaerospermum genome, a common human allergy-eliciting Dothideomycetes. Scientific Reports, 6 (1). p. 27008. ISSN 2045-2322, DOI https://doi.org/10.1038/srep27008.
Full text not available from this repository.Abstract
Cladosporium sphaerospermum, a dematiaceous saprophytic fungus commonly found in diverse environments, has been reported to cause allergy and other occasional diseases in humans. However, its basic biology and genetic information are largely unexplored. A clinical isolate C. sphaerospermum genome, UM 843, was re-sequenced and combined with previously generated sequences to form a model 26.89 Mb genome containing 9,652 predicted genes. Functional annotation on predicted genes suggests the ability of this fungus to degrade carbohydrate and protein complexes. Several putative peptidases responsible for lung tissue hydrolysis were identified. These genes shared high similarity with the Aspergillus peptidases. The UM 843 genome encodes a wide array of proteins involved in the biosynthesis of melanin, siderophores, cladosins and survival in high salinity environment. In addition, a total of 28 genes were predicted to be associated with allergy. Orthologous gene analysis together with 22 other Dothideomycetes showed genes uniquely present in UM 843 that encode four class 1 hydrophobins which may be allergens specific to Cladosporium. The mRNA of these hydrophobins were detected by RT-PCR. The genomic analysis of UM 843 contributes to the understanding of the biology and allergenicity of this widely-prevalent species.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education Malaysia: High Impact Research MoE Grant UM.C/625/1/HIR/MOHE/MED/31 (Account no. H-20001-00-E000070), University of Malaya: Postgraduate Research Grant (PPP) PV051/2012A |
| Uncontrolled Keywords: | Allergen; Fungal protein; Melanin; Peptide hydrolase; Polyketide; Siderophore |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 31 May 2018 03:25 |
| Last Modified: | 31 May 2018 03:25 |
| URI: | http://eprints.um.edu.my/id/eprint/18777 |
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