Optimised conditions for pulsed field gel electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Hasima, N.N. and Saidi, J. and Thong, K.L. and Ansary, A. (2001) Optimised conditions for pulsed field gel electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150. ISSN 0128-7451,

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Abstract

Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200 V, ramped pulsed time of 1-25 s and a run time of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Pasteurella multocida; PFGE; restriction enzymes
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 24 Oct 2023 02:56
Last Modified: 27 Mar 2025 08:42
URI: http://eprints.um.edu.my/id/eprint/18592

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