Applications of ribosomal RNA gene restriction pattern analysis and pulsed-field gel electrophoresis in distinguishing Salmonella welterveden isolates in Malaysia.

Goh, Yee-Ling and Puthucheary, S.D. and Thong, K.L. (2000) Applications of ribosomal RNA gene restriction pattern analysis and pulsed-field gel electrophoresis in distinguishing Salmonella welterveden isolates in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health, 31 (4). pp. 697-701. ISSN 01251562,

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Abstract

A representative sample of 20 isolates of Salmonella weltevredenstrains from stool cultures ofpatients admitted at the University Hospital, Kuala Lumpur, Malaysia were analyzed. All the strains weresusceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetracycline, trimethoprim, gentami-cin and co-trimoxazole. Ribosomal RNA gene restriction pattern analysis of PstI-digested DNA gave threeribotypes while pulsed-field gel electrophoresis (PFGE) analysis of XbaI-digested DNA gave ten distinctprofiles. PFGE was more discriminative than ribotyping in distinguishing the strains. The majority of thestrains analyzed were very closely related with similarity coefficient values ranging from 0.8 to 1.0. BothPFGE and ribotyping could distinguish one of the strains which was obtained from a patient following abone marrow transplant for β-thalassemia major, indicating that this particular strain was unrelated to therest of the strains from patients with acute gastroenteritis Application of ribosomal RNA gene restriction patterns analysis and pulsed-field gel electrophoresis in distinguishing Salmonella weltevreden isolates in Malaysia. Available from: https://www.researchgate.net/publication/11926603_Application_of_ribosomal_RNA_gene_restriction_patterns_analysis_and_pulsed-field_gel_electrophoresis_in_distinguishing_Salmonella_weltevreden_isolates_in_Malaysia [accessed Apr 13 2018].

Item Type: Article
Funders: UNSPECIFIED
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 15 Feb 2022 08:20
Last Modified: 15 Feb 2022 08:20
URI: http://eprints.um.edu.my/id/eprint/18589

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