Separation and identification of bioactive peptides from stem of Tinospora cordifolia (Willd.) Miers

Pachaiappan, R. and Tamboli, E. and Acharya, A. and Su, C.H. and Gopinath, S.C.B. and Chen, Y. and Velusamy, P. (2018) Separation and identification of bioactive peptides from stem of Tinospora cordifolia (Willd.) Miers. PLoS ONE, 13 (3). e0193717. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0193717.

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Abstract

Enzyme hydrolysates (trypsin, papain, pepsin, a-chymotrypsin, and pepsin-pancreatin) of Tinospora cordifolia stem proteins were analyzed for antioxidant efficacy by measuring (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot) radical scavenging activity, (2) 2,20-azinobis(3-ethyl-benzothiazoline- 6-sulfonic acid) (ABTS(+)) radical scavenging capacity, and (3) Fe2+ chelation. Trypsin hydrolysate showed the strongest DPPH center dot scavenging, while alpha-chymotrypsin hydrolysate exhibited the highest ABTS(+) scavenging and Fe2+ chelation. Undigested protein strongly inhibited the gastrointestinal enzymes, trypsin (50% inhibition at enzyme/substrate ratio = 1: 6.9) and a-chymotrypsin (50% inhibition at enzyme/substrate ratio = 1: 1.82), indicating the prolonged antioxidant effect after ingestion. Furthermore, gel filtration purified peptide fractions of papain hydrolysates exhibited a significantly higher ABTS(+) and superoxide radical scavenging as compared to non-purified digests. Active fraction 9 showing the highest radical scavenging ability was further purified and confirmed by MALDI-TOF MS followed by MS/MS with probable dominant peptide sequences identified are VLYSTPVKM-WEPGR, VITVVATAGSETMR, and HIGININSR. The obtained results revealed that free radical scavenging capacity of papain hydrolysates might be related to its consistently low molecular weight hydrophobic peptides.

Item Type:	Article
Funders:	University of Malaya Research grants—“MoE Grant UM.C/625/1/HIR/MOE/DENT/09”, University of Malaya Research Grants (UMRG) RG454-12HTM
Uncontrolled Keywords:	Radical-scavenging activities; In-vitro digests; Antioxidant activity; Protein hydrolysate; Functional-properties; Zein hydrolysate; Liposome system; Possible mode; Fractions; Assay; Science & Technology - Other Topics
Subjects:	R Medicine > RK Dentistry > Practice of dentistry. Dental economics
Divisions:	Faculty of Dentistry > Dept of Oral Biology
Depositing User:	Mr Ahmad Azwan Azman
Date Deposited:	04 Apr 2018 02:27
Last Modified:	04 Apr 2018 02:27
URI:	http://eprints.um.edu.my/id/eprint/18574

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