Aberrant Methylation of Ribonucleotide Reductase Subunit M2 Is Closely Associated with Oral Cancer

Kho, Goot Heah and Froemming, Gabriele Ruth Anisah and Zain, Rosnah B and Thong, Kwai Lin (2016) Aberrant Methylation of Ribonucleotide Reductase Subunit M2 Is Closely Associated with Oral Cancer. Academic Journal of Cancer Research, 9 (4). pp. 63-69. ISSN 1995-8943, DOI https://doi.org/10.5829/idosi.ajcr.2016.63.69.

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Abstract

Gene inactivation by promoter hypermethylation is a common occurrence in the development of various human cancers including oral cancer. Previous evidences observed that DNA methylation play a vital role in the carcinogenesis of oral cancer. The aim of this study was to analyze methylation status and protein expression of RRM2 in oral cancer. Archival tissues of oral cancer and healthy mucosa tissues were retrieved for the study. The methylation status of RRM2 was examined by methylation specific polymerase chain reaction and its protein expression was measured by immunohistochemistry. In the present study, frequency of aberrant methylation of RRM2 was 80% (n=32) in tumour tissues, whereas such methylation status was negatively detected in normal mucosa. In addition, 20 % (n=8) for RRM2 immunoreactivity was revealed in tumour tissues. In tumour tissues, RRM2 promoter hypermethylation was frequently associated with its reduced protein expression. Thus, epigenetic inactivation of RRM2 through promoter hypermethylation may be important in the oral cancer progression and assessment of RRM2 methylation status in oral cancer may become a potential biomarker for early detection in oral cancer.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Oral cancer; Ribonucleotide reductase; Promoter; Methylation
Subjects: Q Science > Q Science (General)
R Medicine
R Medicine > RK Dentistry
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 12 Feb 2018 02:29
Last Modified: 12 Oct 2018 03:26
URI: http://eprints.um.edu.my/id/eprint/18520

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