Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae

Azman, A.S. and Zainal, N. and Mutalib, N.S.A. and Yin, W.F. and Chan, K.G. and Lee, L.H. (2016) Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae. International Journal of Systematic and Evolutionary Microbiology, 66 (2). pp. 554-561. ISSN 1466-5026

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Official URL: https://doi.org/10.1099/ijsem.0.000753

Abstract

A novel actinobacterial strain, MUSC 78T, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78T represented a novel lineage within the class Actinobacteria. Strain MUSC 78T formed a distinct clade in the family Intrasporangiaceae and was related most closely to members of the genera Terrabacter (98.3–96.8 % 16S rRNA gene sequence similarity), Intrasporangium (98.2– 96.8 %), Humibacillus (97.2 %), Janibacter (97.0–95.3 %), Terracoccus (96.8 %), Kribbia (96.6 %), Phycicoccus (96.2–94.7 %), Knoellia (96.1–94.8 %), Tetrasphaera (96.0–94.9 %) and Lapillicoccus (95.9 %). Cells were irregular rod-shaped or cocci and stained Grampositive. The cell-wall peptidoglycan type was A3γ, with LL-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H4). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0.The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78T exhibited phylogenetic and phenotypic differences from members of the genera of the family Intrasporangiaceae, and therefore a novel species of a new genus, Monashia flava gen. nov., sp. nov., is proposed. The type strain of Monashia flava is MUSC 78T (=DSM 29621T=MCCC 1K00454T=NBRC 110749T).

Item Type: Article
Uncontrolled Keywords: Cardiolipin; Diamino acid; Diaminopimelic acid; Fatty acid; Galactose; Glycolipid; Mannose; Menaquinone; Peptidoglycan; Phosphatidylethanolamine; Phosphatidylglycerol; Phosphatidylinositol; Rhamnose; RNA 16S; Sugar
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Dec 2017 03:54
Last Modified: 04 Dec 2017 03:54
URI: http://eprints.um.edu.my/id/eprint/18413

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