Endophytic filamentous fungi from a Catharanthus roseus: Identification and its hydrolytic enzymes

Ayob, F.W. and Simarani, K. (2016) Endophytic filamentous fungi from a Catharanthus roseus: Identification and its hydrolytic enzymes. Saudi Pharmaceutical Journal, 24 (3). pp. 273-278. ISSN 1319-0164

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Official URL: https://doi.org/10.1016/j.jsps.2016.04.019

Abstract

This paper reported on the various filamentous fungi strains that were isolated from a wild grown Catharanthus roseus. Based on the morphological characteristics and molecular technique through a Polymerase Chain Reaction and DNA sequencing method using internal transcribed spacer (ITS), these fungi had been identified as a Colletotrichum sp., Macrophomina phaseolina, Nigrospora sphaerica and Fusarium solani. The ultrastructures of spores and hyphae were observed under a Scanning Electron Microscope. The hydrolytic enzyme test showed that all strains were positive in secreting cellulase. Colletotrichum sp. and F. solani strains also gave a positive result for amylase while only F. solani was capable to secrete protease. These fungi were putatively classified as endophytic fungi since they produced extracellular enzymes that allow them to penetrate plant cell walls and colonize with symbiotic properties.

Item Type: Article
Uncontrolled Keywords: Hydrolytic enzyme; Medicinal plant; Symbiotic microorganisms; Molecular and morphological identification
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 Nov 2017 06:32
Last Modified: 30 Nov 2017 06:32
URI: http://eprints.um.edu.my/id/eprint/18395

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