A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Benacer, D. and Mohd Zain, S.N. and Lewis, J.W. and Khalid, M.K.N.M. and Thong, Kwai Lin (2017) A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. Revista da Sociedade Brasileira de Medicina Tropical, 50 (2). pp. 1-4. ISSN 0037-8682, DOI https://doi.org/10.1590/0037-8682-0364-2016.

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Abstract

INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

Item Type:	Article
Funders:	University of Malaya, Kuala Lumpur, Malaysia: Research grant (RG053/11BIO & RP016B-14AFR) and Malaya High Impact Research Grant (reference UM.C/625/1HIR/MOHE/CHAN/11/2)
Uncontrolled Keywords:	Leptospira; Leptospirosis; Duplex endpoint; PCR
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Prof. Dr Kwai Lin Thong
Date Deposited:	30 Nov 2017 01:27
Last Modified:	15 Oct 2018 05:39
URI:	http://eprints.um.edu.my/id/eprint/18378

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