Protective effect of antioxidant extracts from Grey oyster mushroom, pleurotus pulmonarius (Agaricomycetes), against human low-density lipoprotein oxidation and aortic endothelial cell damage

Abidin, M.H.Z. and Abdullah, N. and Abidin, N.Z. (2016) Protective effect of antioxidant extracts from Grey oyster mushroom, pleurotus pulmonarius (Agaricomycetes), against human low-density lipoprotein oxidation and aortic endothelial cell damage. International Journal of Medicinal Mushrooms, 18 (2). pp. 109-121. ISSN 1521-9437

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Abstract

This study evaluated the in vitro antioxidant capacities of extracts from Pleurotus pulmonarius via Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. Extract compositions were determined by phenol–sulfuric acid; Coomassie Plus (Bradford) protein; Spectroquant zinc, copper, and manganese test assays; and liquid chromatography– tandem mass spectrometry (LC/MS/MS) and gas chromatography–mass spectrometry (GC/MS). Methanol-dichloromethane extract, water fraction, hot water, aqueous extract and hexane fraction exhibited the most potent extracts in the antioxidant activities. LC/MS/MS and GC/MS showed that the extracts contained ergothioneine, ergosterol, flavonoid, and phenolic compounds. The selected potent extracts were evaluated for their inhibitory effect against oxidation of human low-density lipoproteins and protective effects against hydrogen peroxide–induced cytotoxic injury in human aortic endothelial cells. The crude aqueous extract was deemed most potent for the prevention of human low-density lipoprotein oxidation and endothelial membrane damage. Ergothioneine might be the compound responsible for the activities, as supported by previous reports. Thus, P. pulmonarius may be a valuable antioxidant ingredient in functional foods or nutraceuticals.

Item Type: Article
Uncontrolled Keywords: Medicinal mushrooms; Oxidative stress; Antioxidant; Functional food; LDL oxidation; Human aortic endothelial membrane damage
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 13 Oct 2017 03:14
Last Modified: 13 Oct 2017 03:14
URI: http://eprints.um.edu.my/id/eprint/18010

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