Alternative sweeteners influence the biomass of oral biofilm

Razak, Fathilah Abdul and Baharuddin, B.A. and Akbar, E.F.M. and Norizan, A.H. and Ibrahim, N.F. and Yusoff, M. (2017) Alternative sweeteners influence the biomass of oral biofilm. Archives of Oral Biology, 80. pp. 180-184. ISSN 0003-9969, DOI https://doi.org/10.1016/j.archoralbio.2017.04.014.

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Abstract

Objective Compact-structured oral biofilm accumulates acids that upon prolonged exposure to tooth surface, causes demineralisation of enamel. This study aimed to assess the effect of alternative sweeteners Equal Stevia®, Tropicana Slim®, Pal Sweet® and xylitol on the matrix-forming activity of plaque biofilm at both the early and established stages of formation. Methods Saliva-coated glass beads (sGB) were used as substratum for the adhesion of a mixed-bacterial suspension of Streptococcus mutans, Streptococcus sanguinis and Streptococcus mitis. Biofilms formed on sGB at 3 h and 24 h represented the early and established-plaque models. The biofilms were exposed to three doses of the sweeteners (10%), introduced at three intervals to simulate the exposure of dental plaque to sugar during three consecutive food intakes. The treated sGB were (i) examined under the SEM and (ii) collected for turbidity reading. The absorbance indicated the amount of plaque mass produced. Analysis was performed comparative to sucrose as control. Results Higher rate of bacterial adherence was determined during the early compared to established phases of formation. Comparative to the sweeteners, sucrose showed a 40% increase in bacterial adherence and produced 70% more plaque-mass. Bacterial counts and SEM micrographs exhibited absence of matrix in all the sweetener-treated biofilms at the early phase of formation. At the established phase, presence of matrix was detected but at significantly lower degree compared to sucrose (p < 0.05). Conclusion Alternatives sweeteners promoted the formation of oral biofilm with lighter mass and lower bacterial adherence. Hence, suggesting alternative sweeteners as potential antiplaque agents.

Item Type:	Article
Funders:	High Impact Research, Malaysia Ministry of Education (MoE) Grant UM.C/625/1/HIR/MoE/15, University of Malaya Research Grant (RP036-15HTM)
Uncontrolled Keywords:	Oral biofilm; Adhesion; Plaque-mass; Sucrose; Xylitol; Cariogenic; Potential; Streptococcus-mutans; Caries prevention; Erythritol; Metabolism; Sorbitol; Sucrose; Plaque; Dentistry, Oral Surgery & Medicine
Subjects:	R Medicine > RK Dentistry > Practice of dentistry. Dental economics
Divisions:	Faculty of Dentistry > Dept of Oral Biology Faculty of Science > Institute of Biological Sciences
Depositing User:	Mr Ahmad Azwan Azman
Date Deposited:	12 Sep 2017 08:11
Last Modified:	23 Dec 2019 07:29
URI:	http://eprints.um.edu.my/id/eprint/17796

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