Wafa, Sharifah Nurashikin and Taha, Rosna Mat and Mohajer, Sadegh and Mahmad, Noraini and Ahmed, Bakrudeen Ali (2016) Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL. BioMed Research International, 2016. pp. 1-9. ISSN 2314-6133, DOI https://doi.org/10.1155/2016/2820454.
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Abstract
An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya. Postgraduate PPP grant (PV025/2011) |
| Uncontrolled Keywords: | Organogenesis; Ultrastructural Features; In Vitro; Grown; Canna indica L. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 14 Jul 2017 03:02 |
| Last Modified: | 24 Oct 2018 04:08 |
| URI: | http://eprints.um.edu.my/id/eprint/17503 |
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