Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma

Alabsi, A.M. and Lim, K.L. and Paterson, I.C. and Ali-Saeed, R. and Muharram, B.A. (2016) Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International, 2016. pp. 1-13. ISSN 2314-6133, DOI https://doi.org/10.1155/2016/4904016.

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Abstract

Dracaena cinnabari Balf.f. is a red resin endemic to Socotra Island, Yemen. Although there have been several reports on its therapeutic properties, information on its cytotoxicity and anticancer effects is very limited. This study utilized a bioassay-guided fractionation approach to determine the cytotoxic and apoptosis-inducing effects of D. cinnabari on human oral squamous cell carcinoma (OSCC). The cytotoxic effects of D. cinnabari crude extract were observed in a panel of OSCC cell lines and were most pronounced in H400. Only fractions DCc and DCd were active on H400 cells; subfractions DCc15 and DCd16 exhibited the greatest cytotoxicity against H400 cells and D. cinnabari inhibited cells proliferation in a time-dependent manner. This was achieved primarily via apoptosis where externalization of phospholipid phosphatidylserine was observed using DAPI/Annexin V fluorescence double staining mechanism studied through mitochondrial membrane potential assay cytochrome enzyme-linked immunosorbent and caspases activities revealed depolarization of mitochondrial membrane potential (MMP) and significant activation of caspases 9 and 3/7, concomitant with S phase arrest. Apoptotic proteins array suggested that MMP was regulated by Bcl-2 proteins family as results demonstrated an upregulation of Bax, Bad, and Bid as well as downregulation of Bcl-2. Hence, D. cinnabari has the potential to be developed as an anticancer agent.

Item Type:	Article
Funders:	UMRG RP002D-13HTM, RG422/12HTM
Uncontrolled Keywords:	Cell Cycle Arrest; Apoptosis Induction; Modulation; Mitochondrial Integrity; Bcl-2 Family Members; Caspase Dependence; Dracaena cinnabari-Treated; H400; Human Oral; Squamous Cell Carcinoma
Subjects:	R Medicine R Medicine > RK Dentistry
Divisions:	Faculty of Dentistry
Depositing User:	Ms. Juhaida Abd Rahim
Date Deposited:	11 Jul 2017 08:31
Last Modified:	11 Jul 2017 08:31
URI:	http://eprints.um.edu.my/id/eprint/17483

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