Phosphorylated and Nonphosphorylated PfMAP2 Are Localized in the Nucleus, Dependent on the Stage ofPlasmodium falciparumAsexual Maturation

Dahalan, F.A. and Sidek, H.M. and Murtey, M.D. and Embi, M.N. and Ibrahim, J. and Fei Tieng, L. and Zakaria, N.A. and Abdul-Aziz, N.M. (2016) Phosphorylated and Nonphosphorylated PfMAP2 Are Localized in the Nucleus, Dependent on the Stage ofPlasmodium falciparumAsexual Maturation. BioMed Research International, 2016. pp. 1-9. ISSN 2314-6133, DOI https://doi.org/10.1155/2016/1645097.

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Abstract

Plasmodium falciparum mitogen-activated protein (MAP) kinases, a family of enzymes central to signal transduction processes including inflammatory responses, are a promising target for antimalarial drug development. Our study shows for the first time that the P. falciparum specific MAP kinase 2 (PfMAP2) is colocalized in the nucleus of all of the asexual erythrocytic stages of P. falciparum and is particularly elevated in its phosphorylated form. It was also discovered that PfMAP2 is expressed in its highest quantity during the early trophozoite (ring form) stage and significantly reduced in the mature trophozoite and schizont stages. Although the phosphorylated form of the kinase is always more prevalent, its ratio relative to the nonphosphorylated form remained constant irrespective of the parasites’ developmental stage. We have also shown that the TSH motif specifically renders PfMAP2 genetically divergent from the other plasmodial MAP kinase activation sites using Neighbour Joining analysis. Furthermore, TSH motif-specific designed antibody is crucial in determining the location of the expression of the PfMAP2 protein. However, by using immunoelectron microscopy, PPfMAP2 were detected ubiquitously in the parasitized erythrocytes. In summary, PfMAP2 may play a far more important role than previously thought and is a worthy candidate for research as an antimalarial.

Item Type:	Article
Funders:	MOSTI 09-05-IFN-BPH-001, (FRGS-MOHE) FS344/2008C
Uncontrolled Keywords:	Phosphorylated; Nonphosphorylated; PfMAP2; Nucleus; Plasmodium falciparum; Asexual Maturation
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine
Depositing User:	Ms. Juhaida Abd Rahim
Date Deposited:	11 Jul 2017 07:56
Last Modified:	11 Jul 2017 07:56
URI:	http://eprints.um.edu.my/id/eprint/17480

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