Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae)

Yong, Hoi Sen and Song, Sze Looi and Eamsobhana, Praphathip and Goh, Share Yuan and Lim, Phaik Eem (2015) Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae). Acta Tropica, 152. pp. 157-164. ISSN 0001-706X

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Official URL: https://doi.org/10.1016/j.actatropica.2015.09.001

Abstract

Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. Earlier work on its mitochondrial genome was based on long polymerase chain reaction method. To date, only the mitogenome of the isolates from China has been studied. We report here the complete mitogenome of the Thailand isolate based on next generation sequencing and compare the genetic diversity with other isolates. The mitogenome of the Thailand isolate (13,519 bp) is longer than those of the China isolates (13,497-13,502 bp). Five protein-coding genes (atp6, cox1, cox2, cob, nad2) show variations in length among the isolates. The stop codon of the Thailand isolate differs from the China and Taiwan isolates in 4 genes (atp6, cob, nad2, nad6). Additionally, the Thailand isolate has 4 incomplete T stop codon compared to 3 in the China and Taiwan isolates. The control region is longer in the Thailand isolate (258 bp) than the China (230-236 bp) and Taiwan (237 bp) isolates. The intergenic sequence between nad4 and coxl genes in the Thailand isolate lacks 2 bp (indels) at the 5'-end of the sequence as well as differs at 7 other sites compared to the China and Taiwan isolates. In the Thailand isolate, 18 tRNAs lack the entire T Psi C-arm, compared to 17 in the China isolate and 16 in the Taiwan isolate. Phylogenetic analyses based on 36 mt-genes, 12 PCGs, 2 rRNA genes, 22 tRNA genes and control region all indicate closer genetic affinity between the China and Taiwan isolates compared to the Thailand isolate. Based on 36 mt-genes, the inter-isolate genetic distance varies from p =3.2% between China and Taiwan isolates to p = 11.6% between Thailand and China isolates. The mitogenome will be useful for population, phylogenetics and phylogeography studies. (C) 2015 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Angiostrongylus cantonensis; Rat lungworm; Mitochondrial genome; Transfer RNA; Genetic diversity; Phylogenetics
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Faculty of Science > Institute of Biological Sciences
Depositing User: Mrs. Siti Mawarni Salim
Date Deposited: 26 Sep 2016 02:56
Last Modified: 05 Dec 2019 04:33
URI: http://eprints.um.edu.my/id/eprint/16533

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