Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection

Hosseini, S. and Ibrahim, F. and Rothan, H.A. and Yusof, R. and van der Marel, C. and Djordjevic, I. and Koole, L.H. (2015) Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection. Biochemical Engineering Journal, 99. pp. 183-192. ISSN 1369-703X, DOI https://doi.org/10.1016/j.bej.2015.04.001.

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Abstract

Polymethylmethacrylate-co-methacrylic acid, poly(MMA-co-MAA) coatings were produced with different initial molar ratios of monomers (MMA and MAA) in free-radical polymerization reaction. Polymeric platforms were specifically designed with controlled concentration of surface-exposed carboxyl (-COOH) groups that can be used as a desirable functionality for protein immobilization. Spin-coated chips were used for antibody (Ab) immobilization in order to investigate the influence of -COOH surface concentration on dengue virus detection efficiency in enzyme-linked immunosorbent assay (ELISA) experiment. Successful immobilization of Ab was achieved by two different techniques: (1) physical adsorption; and (2) covalent immobilization by carbodiimide coupling between the surface -COOH groups and amine functionalities of dengue Ab molecules. Produced polymer coatings were characterized with surface spectroscopy techniques (Raman and X-ray photoelectron spectroscopy, XPS) and water-in-air contact angle (WCA) measurements. In particular, this research concentrated on the aging effect on the availability and activity of surface -COOH groups. For that reason, WCA and Ab immobilization (ELISA) experiments were repeated on coated biochips after 3,6 and 9 months of storage. Results in this paper describe the robust and sustainable functionalized polymeric platform that can be used effectively for protein activation and development of novel biosensors. (C) 2015 Elsevier B.V. All rights reserved.

Item Type: Article
Funders: University of Malaya from the Ministry of Higher Education Malaysia UM-MOHEUM.C/625/1/HIR/MOHE/05, University of Malaya UMRG: RP009E-13AET
Additional Information: ISI Document Delivery No.: CJ2YF Times Cited: 0 Cited Reference Count: 41 Cited References: Alcon S, 2002, J CLIN MICROBIOL, V40, P376, DOI 10.1128/JCM.40.2.376-381.2002 Audouin F, 2012, BIOMACROMOLECULES, V13, P3787, DOI 10.1021/bm301251r Bai YL, 2006, LANGMUIR, V22, P9458, DOI 10.1021/la061123l Brown L, 2006, LAB CHIP, V6, P66, DOI 10.1039/b512179e Bucatariu F, 2013, APPL SURF SCI, V280, P812, DOI 10.1016/j.apsusc.2013.05.067 Cavalcanti IT, 2012, BIOCHEM ENG J, V67, P225, DOI 10.1016/j.bej.2012.06.016 Chen G, 2012, BIOCHEM ENG J, V67, P120, DOI 10.1016/j.bej.2012.06.002 Coad BR, 2013, SURF COAT TECH, V233, P169, DOI 10.1016/j.surfcoat.2013.05.019 Dong J, 1997, MACROMOLECULES, V30, P286, DOI 10.1021/ma9607168 Dong J, 1997, MACROMOLECULES, V30, P1111, DOI 10.1021/ma960693x DYBAL J, 1990, MACROMOLECULES, V23, P1301, DOI 10.1021/ma00207a013 Goddard JM, 2007, PROG POLYM SCI, V32, P698, DOI 10.1016/j.progpolymsci.2007.04.002 Hosseini S, 2014, EUR POLYM J, V60, P14, DOI 10.1016/j.eurpolymj.2014.08.010 Hosseini S, 2014, ANALYST, V139, P2933, DOI 10.1039/c3an01789c Hosseini S, 2014, APPL SURF SCI, V300, P43, DOI 10.1016/j.apsusc.2014.01.203 Hosseini S, 2014, APPL SURF SCI, V317, P630, DOI 10.1016/j.apsusc.2014.08.167 Ibrahim M, 2005, INDIAN J PURE AP PHY, V43, P911 Kirsch J, 2013, CHEM SOC REV, V42, P8733, DOI 10.1039/c3cs60141b Li B, 2005, BIOMATERIALS, V26, P1487, DOI 10.1016/j.biomaterials.2004.05.004 Linares EM, 2013, BIOSENS BIOELECTRON, V41, P180, DOI 10.1016/j.bios.2012.08.005 Nge TT, 2002, J APPL POLYM SCI, V83, P1025, DOI 10.1002/app.10010 Noro A, 2013, J BIOMED MATER RES B, V101B, P355, DOI 10.1002/jbm.b.32846 Rastian Z, 2014, BIOCHEM ENG J, V90, P16, DOI 10.1016/j.bej.2014.05.009 Sam S., 2009, LANGMUIR, V26, P809, DOI DOI 10.1021/LA902220A Saralidze K, 2007, BIOMATERIALS, V28, P2457, DOI 10.1016/j.biomaterials.2006.12.031 Schilli CM, 2004, MACROMOLECULES, V37, P7861, DOI 10.1021/ma035838w Schmidt SW, 2010, LANGMUIR, V26, P15333, DOI 10.1021/la102435f Shrivastava A., 2011, CHRON YOUNG SCI, V2, P21, DOI DOI 10.4103/2229-5186.79345 Shu PY, 2003, CLIN DIAGN LAB IMMUN, V10, P622, DOI 10.1128/CDLI.10.4.622-630.2003 Situma C, 2005, ANAL BIOCHEM, V340, P123, DOI 10.1016/j.ab.2005.01.044 Song SH, 2012, J BIOMED MATER RES B, V100B, P1289, DOI 10.1002/jbm.b.32695 Stevens AJ, 2009, J MED CHEM, V52, P7911, DOI 10.1021/jm900652e Tennico YH, 2010, SENSOR ACTUAT B-CHEM, V143, P799, DOI 10.1016/j.snb.2009.10.001 Thomas KJ, 2008, J OPT A-PURE APPL OP, V10, DOI 10.1088/1464-4258/10/5/055303 Thomas L, 2010, AM J TROP MED HYG, V83, P696, DOI 10.4269/ajtmh.2010.10-0138, 10.1269/ajtmh.2010.10-0138 Varma HK, 2003, BIOMATERIALS, V24, P297, DOI 10.1016/S0142-9612(02)00318-6 Vesel A, 2012, VACUUM, V86, P634, DOI 10.1016/j.vacuum.2011.07.005 Vesel A, 2012, VACUUM, V86, P773, DOI 10.1016/j.vacuum.2011.07.019 Wang C, 2011, LANGMUIR, V27, P12058, DOI 10.1021/la202267p Yonamine Y, 2012, BIOMACROMOLECULES, V13, P2952, DOI 10.1021/bm300986j Yoon JY, 1996, J COLLOID INTERF SCI, V177, P613, DOI 10.1006/jcis.1996.0075 Hosseini, Samira Ibrahim, Fatimah Rothan, Hussin A. Yusof, Rohana van der Marel, Cees Djordjevic, Ivan Koole, Leo H. Engineering, Faculty /I-7935-2015 Engineering, Faculty /0000-0002-4848-7052 University of Malaya from the Ministry of Higher Education Malaysia UM-MOHEUM.C/625/1/HIR/MOHE/05; University of Malaya UMRG: RP009E-13AET This research is supported by University of Malaya High Impact Research Grant UM-MOHEUM.C/625/1/HIR/MOHE/05 from the Ministry of Higher Education Malaysia and University of Malaya Research Grant (UMRG: RP009E-13AET). Authors would like to acknowledge Mrs. Elham Farahmand for her technical contribution. Authors would also like to acknowledge Mr. Amir Hosseini Hosseini for the graphical design. 0 ELSEVIER SCIENCE BV AMSTERDAM BIOCHEM ENG J
Uncontrolled Keywords: Polymer coatings, surface functional groups, immobilization, enzyme activity, immunoassay, biosensors, linked-immunosorbent-assay, poly(methyl methacrylate), poly(acrylic acid), pmma polymer, nanoparticles, microspheres, fabrication, biosensor, elisa, raman,
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 09 Mar 2016 03:30
Last Modified: 01 Nov 2017 06:07
URI: http://eprints.um.edu.my/id/eprint/15695

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