Oxytetracycline-resistant bacteria in coastal waters in Malaysia

Kian, G.Y. and Chui, W.B. and Choon, W.L. (2012) Oxytetracycline-resistant bacteria in coastal waters in Malaysia. In: 2nd Conference for Regional Cooperation in Ocean and Earth Science Research in the South China Sea, 21-24 October 2012, University of Malaya, Kuala Lumpur, Malaysia.

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Abstract

Oxytetracycline (OTC) is one of the most widely used antibiotics in aquaculture worldwide because of its broad spectrum of activity. The prevalence of OTCresistant environmental bacteria poses a serious risk in human and veterinary medicine. In this study, we isolated 140 and 142 OTC-resistant bacteria (~60Ilg/ml) from Port Dickson (PO) and Port Klang (PK), respectively. Among these OTC-resistant bacteria, 79 isolates (25 from PO and 54 from PK) were highly resistant to OTC (~2401lg!ml). These 79 isolates were then separated into different OTUs by restriction fragment length polymorphism (RFLP) method using three restriction enzymes (Rsal, Cfot, DdeI). The different OTUs were identified via 16S rONA partial sequencing. The dominant OTC-resistant bacteria in both sites were Alteromonas spp., Pseudoalteromonas spp., Shew anella spp. and Vibrio spp. Further testing on the susceptibility of these OTC-resistant bacteria against different classes of antibiotics will be carried out since OTC resistance is known to have cross-resistance to other unrelated antibiotics.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Oxytetracycline (OTC); Malaysia; Antibiotic; Bacteria
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Depositing User: Mr. Mohd Safri
Date Deposited: 14 Dec 2015 07:38
Last Modified: 14 Dec 2015 07:38
URI: http://eprints.um.edu.my/id/eprint/15054

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