Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)

Sakinah, A. and Rosna, M.T. (2015) Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L). In: 17th International Conference on Biotechnology (ICBT 2015 : 17th), 12-13 November 2015, Madrid, Spain.

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Abstract

Antioxidants are compounds that protect cells against the damaging effects of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, and peroxy nitrite which result in oxidative stress leading to cellular damage. Natural antioxidant are in high demand because of their potential in health promotion and disease prevention and their improved safety and consumer acceptability. Plants are rich sources of natural antioxidant. Dioscorea a/ata L. known as "ubi badak" in Malaysia were well known for their antioxidant content but this plant was seasonal. Thus, tissue culture technique was used to mass propagate this plant. In the present work, a comparative study between in vitro (from tissue culture) and in vivo (from intact plant) samples of Dioscorea alata L. for their antioxidant potential by 2,2-diphenil -1- picrylhydrazyl (DPPH) radical scavenging activity method and their total phenolic and flavonoid contents were carried out. All samples had better radical scavenging activity but in vivo samples had the strongest radical scavenging activity compared to in vitro samples. Furthermore, tubers from in vivo samples showed the greatest free radical scavenging effect and comparatively greater phenolic content than in vitro samples.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Dioscorea alata; Tissue culture; Antioxidant; In vivo; In vitro; DPPH
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Depositing User: Mr. Mohd Safri
Date Deposited: 14 Dec 2015 06:21
Last Modified: 14 Dec 2015 06:21
URI: http://eprints.um.edu.my/id/eprint/15051

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