Tan, J.S. and Ramanan, R.N. and Ling, T.C. and Shuhaimi, M. and Ariff, A.B. (2011) Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture. Biochemical Engineering Journal, 58-59. pp. 124-132.
Full text not available from this repository.Abstract
The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-alpha ab (PrIFN-alpha 2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-alpha 2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-alpha 2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-alpha 2b. The maximum yield of PrIFN-alpha 2b in shake flask culture (501.8 mu g/L) and stirred tank bioreactor (578.8 mu g/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-alpha 2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 mu g/L). (C) 2011 Elsevier By. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Subjects: | Q Science > Q Science (General) |
| Depositing User: | MR Faizal II H |
| Date Deposited: | 05 Nov 2015 10:53 |
| Last Modified: | 05 Nov 2015 10:53 |
| URI: | http://eprints.um.edu.my/id/eprint/14545 |
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