Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection

Alasil, S.M. and Omar, R. and Ismail, S. and Yusof, M.Y. (2015) Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection. International Journal of Bacteriology, 2015. pp. 1-16. ISSN 2356-6957

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Official URL: http://www.hindawi.com/journals/ijb/2015/671562/ab...

Abstract

Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory effects on the QS-controlled virulence factors and biofilm formation of Pseudomonas aeruginosa both in vitro and in vivo. The Paenibacillus sp. culture extract was used to test its anti-QS effects on the LasA protease, LasB elastase, pyoverdin production, and biofilm formation of P. aeruginosa as well as evaluate its therapeutic effects on lung bacteriology, pathology, hematological profile, and serum antibody responses of experimental animals in a rat model of chronic lung infection. Results showed significant decrease in the activities of QS-controlled LasA protease, LasB elastase pyoverdin, and biofilm formation of P. aeruginosa caused by the culture extract. Moreover, the extract significantly prolonged the survival times of rats and facilitated the clearance of biofilm infections from infected lungs. In conclusion, the antiquorum sensing effects of culture extract from a novel species of Paenibacillus provide new insights to combat biofilm-associated infections.

Item Type: Article
Uncontrolled Keywords: Inhibition; Quorum Sensing-Controlled Virulence; Biofilm Formation; Pseudomonas aeruginosa; Culture Extract; Novel Bacterial Species; Paenibacillus; Rat Model; Chronic Lung Infection
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Nov 2015 07:58
Last Modified: 30 Dec 2015 03:45
URI: http://eprints.um.edu.my/id/eprint/14505

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