Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody

Eamsobhana, Praphathip and Mak, J.W. and Yong, Hoi Sen (1997) Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody. Southeast Asian Journal of Tropical Medicine and Public Health, 28 (Spp. 1). pp. 139-142. ISSN 0125-1562

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Official URL: http://www.tm.mahidol.ac.th/seameo/1997-28-1-suppl...

Abstract

A specific monoclonal antibody (AW-3C2) as revealed by ELISA was produced against the adult worm antigens of Parastrongylus cantonensis and used in a sandwich ELISA for the detection of circulating antigens in the sera of parastrongyliasis patients and those with other parasitic diseases. A total of 60 sera was used in this study. Of these, 10 each were from patients with parastrongyliasis filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 serum samples from normal healthy Thais and Malaysians. The mean +/- optical density (OD) values for the normal Thai and Malaysian groups were 0.126 +/- 0.028 and 0.124 +/- 0.029, respectively. Mean OD values of parastrongyliasis patient group differed significantly from that of the normal groups as well as those of other parasitic infections. Using a cut-off point of mean OD +/- 3SD of the normal control groups as indicating a positive reading, the specificity of the assay with this monoclonal antibody was 100% while the sensitivity was 50%.

Item Type: Article
Uncontrolled Keywords: Circulating antigens; Parastrongylus cantonensis; Human sera; Sandwich ELISA; Monoclonal antibody
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 08 May 2015 06:38
Last Modified: 05 Dec 2019 04:12
URI: http://eprints.um.edu.my/id/eprint/13457

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