Immunoblot analysis of antigens from Parastrongylus cantonensis, P. costaricensis and P. malaysiensis using serum antibodies against P. cantonensis

Eamsobhana, P. and Yong, H.S. and Ambu, S. (2000) Immunoblot analysis of antigens from Parastrongylus cantonensis, P. costaricensis and P. malaysiensis using serum antibodies against P. cantonensis. Japanese Journal of Tropical Medicine and Hygiene, 28 (2). pp. 115-119. ISSN 0304-2146

Full text not available from this repository.
Official URL: https://www.jstage.jst.go.jp/article/tmh1973/28/2/...

Abstract

Parastrongylus cantonensis, P. costaricensis and P. malaysiensis adult worm antigens were tested for cross-reactivities in immunoblots using sera from patients with parasitologically confirmed parastrongyliasis. Sera from P. cantonensis-infected patients with a high specificity for the 31-kDa diagnostic P. cantonensis antigen cross-reacted with a corresponding component found in P. costaricensis and P. malaysiensis. The immune sera consistently recognized a prominent immunogenic band with a MW of 31 kDa against the antigens prepared from the 3 Parastrongylus species. Sera obtained from patients infected with Gnathostoma spinigerum and normal healthy control sera did not react with the 31-kDa protein from the 3 species of Parastrongylus. The cross-reactivity observed in the immunoblots performed on serum samples from patients with P. cantonensis infection indicates that the 3 Parastrongylus species share the specific 31-kDa antigen. This protein may be used for the immunodiagnosis of human infection with P. costaricensis and P. malaysiensis.

Item Type: Article
Uncontrolled Keywords: Parastrongylus (= Angiostrongylus) antigens; Immunoblotting; Immunodiagnosis; P. cantonen-sis; P. costaricensis; P. malaysiensis
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 27 Apr 2015 06:51
Last Modified: 27 Apr 2015 06:51
URI: http://eprints.um.edu.my/id/eprint/13398

Actions (login required)

View Item View Item