DNA-based characterisation and classification of forensically important flesh flies (Diptera: Sarcophagidae) in Malaysia

Tan, S.H. and Rizman-Idid, M. and Mohd-Aris, E. and Kurahashi, H. and Mohamed, Z. (2010) DNA-based characterisation and classification of forensically important flesh flies (Diptera: Sarcophagidae) in Malaysia. Forensic Science International, 199 (1-3). pp. 43-49.

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Abstract

Insect larvae and adult insects found on human corpses provide important clues for the estimation of the postmortem interval (PMI). Among all necrophagous insects, flesh flies (Diptera: Sarcophagidae) are considered as carrion flies of forensic importance. DNA variations of 17 Malaysian, two Indonesian and one Japanese flesh fly species are analysed using the mitochondrial COI and COII. These two DNA regions were useful for identifying most species experimented. However, characterisation of the species was not sufficiently made in the case of Sarcophaga javanica. Seventeen Malaysian species of forensic importance were successfully clustered into distinct clades and grouped into the six species groups: peregrina, albiceps, dux, pattoni, princeps and ruficornis. These groups correspond with generic or subgeneric taxa of the subfamily Sarcophaginae: Boettcherisca, Parasarcophaga, Liosarcophaga, Sarcorohdendorfia-Lioproctia, Harpagophalla-Seniorwhitea and Liopygia. The genetic variations found in COI and COII can be applied not only to identify the species of forensic importance, but also to understand the taxonomic positions, generic or subgeneric status, of the sarcophagine species. (c) 2010 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Sarcophagidae; Cytochrome oxidase; DNA-based identification; Flesh flies; Malaysia; Forensic entomology
Subjects: R Medicine
Depositing User: Mr Faizal 2
Date Deposited: 07 Apr 2015 08:45
Last Modified: 07 Apr 2015 08:45
URI: http://eprints.um.edu.my/id/eprint/13227

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