Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense

Al-Obaidi, J.R. and Mohd-Yusuf, Yusmin and Razali, N. and Jayapalan, J.J. and Chin-Chong, T. and Md-Noh, N. and Junit, Sarni Mat and Othman, Rofina Yasmin and Hashim, Onn Haji (2014) Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense. International Journal of Molecular Sciences, 15 (3). pp. 51755-5192. ISSN 1661-6596

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Official URL: http://www.mdpi.com/1422-0067/15/3/5175

Abstract

Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.

Item Type: Article
Uncontrolled Keywords: Basal stem rot; Ganoderma boninense; Biomarker; Oil palm; Two-dimensional gel electrophoresis; ESI-TRAP
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 24 Jan 2015 03:06
Last Modified: 26 Oct 2019 04:43
URI: http://eprints.um.edu.my/id/eprint/12376

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