Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua

Low, V.L. and Tan, T.K. and Domingues, L.N. and Tay, S.T. and Lim, Y.A.L. and Goh, T.G. and Panchadcharam, C. and Bathmanaban, P. and Sofian-Azirun, M. (2014) Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua. Veterinary Parasitology, 204 (3-4). pp. 439-442. ISSN 0304-4017

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Abstract

A multilocus sequence analysis using mitochondria-encoded cytochrome c oxidase subunit I (COI), cytochrome B (CytB), NADH dehydrogenase subunit 5 (ND5); nuclear encoded 18S ribosomal RNA (18S) and 28S ribosomal RNA (28S) genes was performed to determine the levels of genetic variation between the closely related species Haematobia irritans Linnaeus and Haematobia exigua de Meijere. Among these five genes, ND5 and CytB genes were found to be more variable and informative in resolving the interspecific relationships of both species. In contrast, the COI gene was more valuable in inferring the intraspecific relationships. The ribosomal 18S and 28S sequences of H. irritans and H. exigua were highly conserved with limited intra- and inter-specific variation. Molecular evidence presented in this study demonstrated that both flies are genetically distinct and could be differentiated based on sequence analysis of mitochondrial genes.

Item Type: Article
Uncontrolled Keywords: Horn fly; Buffalo fly; Molecular markers; COI; CytB; ND5
Subjects: R Medicine
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 15 Jan 2015 04:57
Last Modified: 15 Jan 2015 04:57
URI: http://eprints.um.edu.my/id/eprint/12090

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