Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources.

Shuan Ju Teh, C.; Lin Thong, K.; Tein Ngoi, S.; Ahmad, N.; Balakrish Nair, G.; Ramamurthy, T. (2009) Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources. The Journal of general and applied microbiology, 55 (6). pp. 419-25. ISSN 0022-1260

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Abstract

A pair of primers targeting the hlyA gene for Vibrio cholerae which could distinguish the classical from El Tor biotypes was designed and combined with other specific primers for ompW, rfb complex, and virulence genes such as ctxA, toxR, and tcpI in a multiplex PCR (m-PCR) assay. This m-PCR correctly identified 39 V. cholerae from clinical, water and seafood samples. The efficiency of this multiplex PCR (m-PCR) was compared with conventional biochemical and serogrouping methods. One O139 and 25 O1 V. cholerae strains including 10 environmental strains harbored all virulence-associated genes except 1 clinical strain which only had toxR and hlyA genes. Thirteen environmental strains were classified as non-O1/non-O139 and had the toxR and hlyA genes only. The detection limit of m-PCR was 7 x 10(4) cfu/ml. The m-PCR test was reliable and rapid and reduced the identification time to 4 h.

Item Type: Article
Creators:
  1. Shuan Ju Teh, C.
  2. Lin Thong, K.
  3. Tein Ngoi, S.
  4. Ahmad, N.
  5. Balakrish Nair, G.
  6. Ramamurthy, T.
Journal or Publication Title: The Journal of general and applied microbiology
Additional Information: Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
Uncontrolled Keywords: Amino Acid Sequence; Animals
Subjects: R Medicine
Divisions: Faculty of Medicine > School of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 06 May 2011 12:22
Last Modified: 06 May 2011 12:22
URI: http://eprints.um.edu.my/id/eprint/1208

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