Yokoyama, H. and Yanagida, T. and Freeman, M.A. and Katagiri, T. and Hosokawa, A. and Endo, M. and Hirai, M. and Takagi, S. (2010) Molecular diagnosis of Myxobolus spirosulcatus associated with encephalomyelitis of cultured yellowtail, Seriola quinqueradiata Temminck & Schlegel. Journal of Fish Diseases, 33 (12). pp. 939-946. ISSN 0140-7775,
Full text not available from this repository.Abstract
Mass mortality of cultured yellowtail, Seriola quinqueradiata, has recently been reported from fish farms in western Japan. Previous studies revealed that diseased fish were characterized by encephalomyelitis and presporogonic stages of a myxosporean-like parasite in the spinal cord. However, the parasite has remained unidentified because of the lack of mature stages being present. Thus, in the present study, analysis of the small subunit ribosomal DNA (18S rDNA) of the parasite as well as in situ hybridization (ISH) studies using histological sections of the infected tissue was conducted. The 18S rDNA of the myxosporean had higher sequence similarities with those of bile-duct-infecting myxosporeans rather than those infecting nervous tissues and was identified as Myxobolus spirosulcatus. The ISH using specific probes demonstrated that the DNA amplified was derived from the multinuclear organisms found in histological sections. A highly sensitive and specific PCR-based assay for M. spirosulcatus was developed, which revealed a high prevalence of infection in cultured yellowtail that exhibited the clinical signs of encephalomyelitis.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Encephalomyelitis; Myxobolus spirosulcatus; myxosporean; Seriola quinqueradiata; yellowtail |
| Subjects: | Q Science > Q Science (General) |
| Depositing User: | Mr Faizal 2 |
| Date Deposited: | 12 Jan 2015 03:59 |
| Last Modified: | 08 Jul 2017 06:53 |
| URI: | http://eprints.um.edu.my/id/eprint/11875 |
Actions (login required)
 |
View Item |
