Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

Vasanthan, P. and Gnanasegaran, N. and Govindasamy, V. and Abdullah, A.N. and Jayaraman, P. and Ronald, V.S. and Musa, S. and Abu Kasim, N.H. (2014) Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells. Biochemical Engineering Journal. ISSN 1369-703X

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Abstract

The scarcity of organs for liver transplant is a major pressure point of liver transplantation. Hence, generating hepatocytes may provide an alternative choice for therapeutic applications. At present, dental pulp stem cell (SCDs) is an emerging source in regenerative medicine. However, existing protocols for cell culture requires fetal bovine serum (FBS) as a nutritional supplement and may carry the risk of transmitting diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in terms of proliferation and differentiation of SCDs into hepatic lineage cells. The result showed that HPL had displayed a superior effect on the proliferation of SCDs. Next, we induced SCDs into hepatic lineage cells which thrived by initiation and followed by maturation into functional hepatocytes for a total of 21 days. We observed that the gene, protein and its functional profile during this differentiation process reiterated in vivo liver development demonstrating a steady down-regulation of early endoderm markers (GATA4, GATA6, SOX17,

Item Type: Article
Additional Information: Doi:10.1016/j.bej.2014.04.007
Uncontrolled Keywords: Endoderm; progenitors; autologous; allogeneic; cell transplantation; liver failure; hepatocyte transplantation; in-vitro; liver; tissue; pluripotency; expression; expansion; culture
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 20 Nov 2014 01:42
Last Modified: 20 Nov 2014 01:42
URI: http://eprints.um.edu.my/id/eprint/11368

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