Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia

Lim, K.; Yasin, R.; Yeo, C.; Puthucheary, S.; Thong, K.L. (2009) Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia. Journal of biomedicine & biotechnology, 2009. p. 165637. ISSN 1110-7251

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    The emergence of Escherichia coli that produce extended spectrum beta-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E. coli harbored the blaTEM gene. Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M. Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron-encoded intI1 integrase being the majority. Amplification and sequence analysis of the 5'CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E. coli isolates.

    Item Type: Article
    1. Lim, K.
    2. Yasin, R.
    3. Yeo, C.
    4. Puthucheary, S.
    5. Thong, K.L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Journal of biomedicine & biotechnology
    Additional Information: Microbiology Division, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Uncontrolled Keywords: Anti-Bacterial Agents/pharmacology
    Subjects: R Medicine
    Divisions: Faculty of Science
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 29 Apr 2011 17:49
    Last Modified: 15 Dec 2014 14:31

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