DNA typing of Calliphorids collected from human corpses in Malaysia

Kavitha, R. and Tan, T.C. and Lee, H.L. and Nazni, W.A. and Sofian-Azirun, Mohd (2013) DNA typing of Calliphorids collected from human corpses in Malaysia. Tropical Biomedicine, 30 (1). pp. 119-124. ISSN 0127-5720

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Abstract

Estimation of post-mortem interval (PMI) is crucial for time of death determination. The advent of DNA-based identification techniques forensic entomology saw the beginning of a proliferation of molecular studies into forensically important Calliphoridae (Diptera). The use of DNA to characterise morphologically indistinguishable immature calliphorids was recognised as a valuable molecular tool with enormous practical utility. The local entomofauna in most cases is important for the examination of entomological evidences. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different insect species on corpses. In this study, calliphorids were collected from 13 human corpses recovered from indoors, outdoors and aquatic conditions during the post-mortem examination by pathologists from the government hospitals in Malaysia. Only two species, Chrysomya megacephala and Chrysomya rufifacies were recovered from human corpses. DNA sequencing was performed to study the mitochondrial encoded COI gene and to evaluate the suitability of the 1300 base pairs of COI fragments for identification of blow fly species collected from real crime scene. The COI gene from blow fly specimens were sequenced and deposited in GenBank to expand local databases. The sequenced COI gene was useful in identifying calliphorids retrieved from human corpses.

Item Type: Article
Uncontrolled Keywords: Mitochondrial-dna oxidase-i postmortem interval blowflies diptera identification sequence carrion cytochrome c oxidase mitochondrial DNA animal article cadaver classification Diptera DNA fingerprinting DNA sequence female forensic medicine genetics growth, development and aging human Malaysia male methodology molecular genetics protein subunit zoology Animals DNA, Mitochondrial Electron Transport Complex IV Entomology Humans Molecular Sequence Data Protein Subunits Sequence Analysis, DNA
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 13 Jul 2014 06:18
Last Modified: 04 Dec 2019 09:20
URI: http://eprints.um.edu.my/id/eprint/10935

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