Studies on plant regeneration and somaclonal variation in Saintpaulia ionantha Wendl. (African Violet)

Daud, N. and Taha, R.M. and Hasbullah, N.A. (2008) Studies on plant regeneration and somaclonal variation in Saintpaulia ionantha Wendl. (African Violet). Pakistan Journal of Biological Sciences, 11 (9). pp. 1240-1245. ISSN 1028-8880

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Abstract

Efficient plant regeneration of Saintpaulia ionantha (African violet) has been obtained in the present study. MS medium supplemented with 1.0 mg L -1 IAA and 2.0 mg L-1 Zeatin resulted in 100 shoot regeneration and induced the highest number of shoots (average 15.0±0.8 shoots per explant) after being cultured for 8 weeks. The above hormone combination was optimum for shoot regeneration. Most of Saintpaulia ionantha plantlets derived from tissue culture system could be hardened and transferred to the greenhouse conditions with 84.0±1.6 success rate. However, regenerated plantlets of Saintpaulia ionantha (even after 12 months old) failed to flower. Morphological characters of regenerated plantlets of Saintpaulia ionantha were observed and compared with in vivo (intact) plants. Regenerated plantlets showed some differences in morphological characters, such as height and leaf size, texture and colour, but the plantlets showed no variation in leaf arrangement and leaf margin. However, the morphological characters of the regenerated plantlets were found to be unstable. © 2008 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Morphogenesis Organogenesis Plantlets Saintpaulia ionantha Somaclonal variation Vegetative propagation indoleacetic acid derivative phytohormone angiosperm article genetics histology metabolism physiology regeneration Angiosperms Indoleacetic Acids Plant Growth Regulators
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 15 Oct 2014 00:59
Last Modified: 15 Oct 2014 00:59
URI: http://eprints.um.edu.my/id/eprint/10771

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