Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of brassica oleracea Var. Botrytis (Cauliflower)

Siong, P.K. and Taha, R.M. and Rahiman, F.A. (2011) Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of brassica oleracea Var. Botrytis (Cauliflower). Acta Biologica Cracoviensia Series Botanica, 53 (1). pp. 26-31. ISSN 0001-5296

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Abstract

We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis On MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19). The number of embryos produced was nigh. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.

Item Type: Article
Uncontrolled Keywords: Somatic embryogenesis cauliflower Brassica oleracea var. botrytis embryogenic callus tissue culture in-vitro propagation culture growth l. transformation protoplasts stability
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 01:03
Last Modified: 20 Aug 2014 01:03
URI: http://eprints.um.edu.my/id/eprint/10768

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