Production of artificial seeds derived from encapsulated in vitro micro shoots of cauliflower, Brassica oleracea var. botrytis

Siong, P.K. and Mohajer, S. and Taha, R.M. (2012) Production of artificial seeds derived from encapsulated in vitro micro shoots of cauliflower, Brassica oleracea var. botrytis. Romanian Biotechnological Letters, 17 (4). pp. 7549-7556. ISSN 1224-5984

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Production_of_artificial_seeds_derived_from_encapsulated_in_vitro_micro_shoots_of_cauliflower,_Brassica_oleracea_var._Botrytis.pdf

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Abstract

A high number of micro shoots (21 +/- 2.31) of Brassica oleracea var. botrytis (cauliflower) were obtained when hypocotyl explants from 2-week-old aseptic seedlings were cultured on MS medium supplemented with 0.1 mg/L NAA and 5 mg/L BAP. Artificial or synthetic seeds were formed when the micro shoots were encapsulated in 4 (w/v) sodium alginate with 100 mM CaCl2 as complexing solution. The artificial seeds took 12 days (after 7 days storage) and 14 days (after 30 days storage) to germinate on MS basal medium. The germination percentage of artificial seeds was enhanced by the inclusion of 0.3 mg/L NAA and 3.0 mg/L BAP in the encapsulation matrix after 7 and 30 days of pre-germination storage. The time taken for germination was also faster (5 days after 7 days of storage and 11 days after 30 days of storage) when MS fortified with 0.3 mg/L NAA and 3.0mg/L BAP were used. Isolated micro shoots encapsulated in MS supplemented with 0.3 mg/L NAA and 3.0mg/L BAP gave high germination percentages (70 +/- 5.43 and 63.33 +/- 4.17) after 7 and 30 days of pre-germination storage period, respectively. Complete plant regeneration was achieved from the germination of these artificial seeds derived from the micro shoots after 3-4 weeks in culture.

Item Type: Article
Uncontrolled Keywords: Synthetic seeds multiple shoots hypocotyl explants MS medium germination micropropagated buds cultures embryos tips
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 00:58
Last Modified: 20 Aug 2014 00:58
URI: http://eprints.um.edu.my/id/eprint/10762

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