Potential of alternative gelling agents in media for the in vitro micro-propagation of Celosia sp

Daud, N. and Taha, R.M. and Noor, N.N.M. and Alimon, H. (2011) Potential of alternative gelling agents in media for the in vitro micro-propagation of Celosia sp. International Journal of Botany, 7 (2). pp. 183-188. ISSN 1811-9700

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Abstract

Plant tissue culture techniques often require optimization for cost reducing by substitute the culture medium with kitchen necessity. This study conducted to investigate various commercial starches namely; cassava flour, rice flour, corn flour and potato starch to be alternatives gelling agents in culture medium preparation. MS basal medium was prepared without plant regulator supplemented with different combinations of alternative gelling agent with Oxoid technical agar. After 8 weeks in culture, the highest of shoot regeneration were obtained on cultured in MS medium containing 40 g L -1 corn flour (10.2Q±9.17), 60 g L -1 cassava flour (40.0Q±6.73), 40 g L 1 rice flour (16:00±12.45) and the combination of 40 g L 1 potato starch plus 2 g L 1 agar (27.20±8.59). ANOVA test shows there were significant differences interactions effects (p<0.05) in terms of number of shoot regenerated between the gelling agents used (40, 50 and 100 g L -1) in combination of agar. On the other hand, no significant different interaction effects at combination of 60 g L -1 gelling agent. The results showed that the combination of alternative gelling agent with agar or gelling agent alone successfully regenerated shoots from the stem segment explants of Celosia sp. No physiological effects were observed on shoots regeneration on Celosia sp. Applications of these alternative gelling agents in tissue culture media should be considered to achieve the optimum benefit for in vitro shoots regeneration. These options are cheaper than control one and could be used for low cost alternatives. © 2011 Asian Network for Scientific Information.

Item Type: Article
Uncontrolled Keywords: Alternative gelling agent Cassava flour Corn flour Potato starch Rice flour Shoot regeneration Celosia Manihot esculenta Solanum tuberosum Zea mays
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 00:57
Last Modified: 20 Aug 2014 00:57
URI: http://eprints.um.edu.my/id/eprint/10760

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