Plant regeneration and floral bud formation from intact floral parts of African violet (Saintpaulia ionantha H. Wendl.) cultured in vitro

Daud, N. and Taha, R.M. (2008) Plant regeneration and floral bud formation from intact floral parts of African violet (Saintpaulia ionantha H. Wendl.) cultured in vitro. Pakistan Journal of Biological Sciences, 11 (7). pp. 1055-1058. ISSN 1028-8880

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Abstract

Intact immature flower buds of African violet (Saintpaulia ionantha II. Wendl.) were used as explant sources for in vitro studies. The effect of exogenous hormones, NAA and BAP on the indirect organogenesis of this species was observed. Callus was formed on the cut end (base) of pedicels of floral buds where they were in contact with the medium. When maintained on the same medium, callus was differentiated into adventitious shoots after 10 weeks in culture. MS media supplemented with 2.0 mg L-1 NAA and 1.0 mg L-1 BAP gave the highest number of sterile or vegetative floral buds from the surface of callus of the explants, but these buds failed to develop further. The floral buds were expanded as abnormal flowers. The floral structures were smaller in size compared to intact flowers. Petals (corolla) were white to purple in colour but did not form any reproductive organs i.e., stamens or pistils. All sterile or vegetative floral buds and abnormal flowers survived for 3 months in culture but failed to reach anthesis. © 2008 Asian Network for Scientific Information.

Item Type: Article
Uncontrolled Keywords: Adventitious shoots In vitro Organogenesis Saintpaulia ionantha Vegetative floral buds 1 naphthylacetic acid 1-naphthaleneacetic acid arylacetic acid derivative benzyl derivative benzyladenine phytohormone purine derivative angiosperm article culture medium drug effect flower growth, development and aging histology physiology regeneration tissue culture technique Angiosperms Benzyl Compounds Culture Media Flowers Naphthaleneacetic Acids Plant Growth Regulators Purines Tissue Culture Techniques
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 01:11
Last Modified: 20 Aug 2014 01:11
URI: http://eprints.um.edu.my/id/eprint/10757

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