Physiological responses of callus from gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation

Hasbullah, N.A. and Taha, R.M. and Saleh, A. and Mohamed, N. (2012) Physiological responses of callus from gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation. Brazilian Archives of Biology and Technology, 55 (3). pp. 411-416. ISSN 1516-8913

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Abstract

In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period.

Item Type: Article
Uncontrolled Keywords: Gerbera jamesonii gamma irradiation callus in vitro chlorophyll soluble protein ultraviolet-b radiation protein growth pigments cultures plants seed RNA
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 01:12
Last Modified: 20 Aug 2014 01:12
URI: http://eprints.um.edu.my/id/eprint/10755

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