Induction of somatic embryogenesis and plant regeneration in Begonia x hiemalis Fotsch. in vitro

Awal, A. and Taha, R.M. and Hasbullah, N.A. (2008) Induction of somatic embryogenesis and plant regeneration in Begonia x hiemalis Fotsch. in vitro. Journal of Biological Sciences, 8 (5). pp. 920-924. ISSN 1727-3048

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Abstract

Direct somatic embryogenesis induction of Begonia x hiemalis Fotsch. (Elatior Begonia) was initiated from two different explants i.e., leaves and petioles. Both explants were cultured on MS medium supplemented with different concentrations of Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D). The results showed that combinations of 0.5-1.0 mg L-1 BAP and 0.1 mg L-1 2,4-D produced direct somatic embryogenesis from leaf and petiole explants. Different concentrations of casein hydrolysate were also tested to optimize somatic embryo induction. The results showed that 100 mg L-1 casein hydrolysate could produce 53.08 nodular callus and 24.16 green embryogenic callus, whereas 500 mg L-1 casein hydrolysate produced 30.83 nodular callus and 23.75 green embryogenic callus. The embryogenic callus were then transferred to MS medium supplemented with 0.5 mg L-1 Gibberelic Acid (GA3) with 0.2 g L-1 activated charcoal for further embryogenesis development and further regeneration. © 2008 Asian Network for Scientific Information.

Item Type: Article
Uncontrolled Keywords: Activated charcoal Begonia � hiemalis fotsch Cascin hydrolysate Direct somatic embryogenesis 2,4 dichlorophenoxyacetic acid activated carbon benzyladenine casein hydrolysate gibberellic acid purine derivative unclassified drug article Begoniaceae callus culture cell culture controlled study culture medium embryo development explant in vitro study nonhuman petiole plant leaf plant regeneration somatic embryogenesis Begonia Begonia hiemalis Elatior
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science
Depositing User: miss munirah saadom
Date Deposited: 20 Aug 2014 01:18
Last Modified: 20 Aug 2014 01:18
URI: http://eprints.um.edu.my/id/eprint/10745

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