In Vitro plant regeneration, antioxidant and antibacterial studies On broccoli, Brassica Oleracea Var. Italica

Farzinebrahimi, R. and Taha, R.M. and Fadainasaband, M. and Mokhtari, S. (2012) In Vitro plant regeneration, antioxidant and antibacterial studies On broccoli, Brassica Oleracea Var. Italica. Pakistan Journal of Botany, 44 (6). pp. 2117-2122. ISSN 0556-3321

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Abstract

Leaf and shoot tip explant of Brassica oleracea var. italica were inoculated on Murashige and Skoog (MS) basal medium supplemented with various concentrations of (BAP) and (IBA), alone and in combinations to achieve plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from In vitro and In vivo plant parts. The highest mean numbers of shoot was observed on combinations of 1 mg l(-1) and 1.5 mg l(-1) of BAP and IBA, 9.49 and 8.69, respectively. The highest number of shoot produced (11.55) per shoot tip explant was recorded on 2.5 mg l(-1) BAP. In addition, highest percentage of roots (100) and highest mean number of roots produced per leaf explant (8.47) occurred on medium with combination of 1.5 mg l(-1) IBA and BAP by shoot tip explant (4.51). The highest antioxidant activity (70) and 2 mm inhibition zone in antibacterial activity were observed in leaf extract compared to Callus extract.

Item Type: Article
Uncontrolled Keywords: Ethanolic extracts, growth, cultures,
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science
Depositing User: miss munirah saadom
Date Deposited: 06 Aug 2014 00:23
Last Modified: 06 Aug 2014 00:23
URI: http://eprints.um.edu.my/id/eprint/10741

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