Effect of picloram, additives and plant growth regulators on somatic embryogenesis of phyla nodiflora (L.) greene

Ahmed, A.B.A.; Rao, A.S.; Rao, M.V.; Taha, R.M. (2011) Effect of picloram, additives and plant growth regulators on somatic embryogenesis of phyla nodiflora (L.) greene. Brazilian Archives of Biology and Technology, 54 (1). pp. 7-13. ISSN 1516-8913

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    Abstract

    The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid (100 mg/L) on 6th subcultures. Further development stages such as torpedo and cotyledonary stage embryos and fostered maturation of embryos were observed at 8(th) and 10(th) subculture. However, the high frequency embryo germination and plantlet (45 plants/20 mg cotyledonary stages embryos) formation was obtained in half-strength MS medium without growth regulators from cotyledonary embryos. All the plantlets established in the field exhibited morphological characters similar to those of the mother plant.

    Item Type: Article
    Creators:
    1. Ahmed, A.B.A.
    2. Rao, A.S.
    3. Rao, M.V.
    4. Taha, R.M.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Brazilian Archives of Biology and Technology
    Uncontrolled Keywords: Somatic embryogenesis (SEs), picloram, ascorbic acid, coconut milk plant growth regulators, Murashige and Skoog medium, suspension-cultures, regeneration, micropropagation, halleridone, protocol,
    Subjects: Q Science > QH Natural history > QH301 Biology
    Divisions: Faculty of Science
    Depositing User: miss munirah saadom
    Date Deposited: 21 Jul 2014 09:14
    Last Modified: 21 Jul 2014 09:14
    URI: http://eprints.um.edu.my/id/eprint/10724

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