Cellular behavior in embryogenic and non-embryogenic sugar beet calluses

Moghaddam, B.E. and Taha, R.M. (2005) Cellular behavior in embryogenic and non-embryogenic sugar beet calluses. In Vitro Cellular and Developmental Biology - Plant, 41 (4). pp. 465-469. ISSN 1054-5476

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Abstract

The present study discusses the results of cytological studies of two kinds of sugar beet callus, i.e., embryogenic and non-embryogenic tissues. The calluses were produced through culture of secondary leaves on Murashige and Skoog medium containing two hormone combinations. One week after transfer of calluses onto fresh medium, their cells were viewed using electron microscopy and an image analyzer. Observations showed that cells of the two callus types had considerable differences in cell structure and various organelles. Of note were the high amount of polyploidization, rough endoplasmic reticulum, polysome, poly-nucleolus, and incomplete cell wall together with abnormal partitioning in non-embryogenic cells, as compared to embryogenic cells. In contrast, vacuolation of cytoplasm, perfect cell wall and partitioning structure, and the high proportion of nucleus/cytoplasm area were recognized in embryogenic cells.

Item Type: Article
Uncontrolled Keywords: Beta vulgaris L. electron microscopy image analysis somatic embryo ultrastructure beta-vulgaris-l neoplastic progression somatic embryogenesis plant-regeneration cells growth calli l.
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science
Depositing User: miss munirah saadom
Date Deposited: 26 Jun 2014 08:33
Last Modified: 26 Jun 2014 08:33
URI: http://eprints.um.edu.my/id/eprint/10712

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